2.10 describe experiments to investigate how enzyme activity can be affected by changes in temperature

2.10 •measure how fast a product appears

                  •the enzyme catalase catalyses the breakdown of hydrogen peroxide into water and oxygen 

                  •collect the oxygen produced and measure how much is given off at a time 

                            •run multiple experiments with the water bath at different temperatures

                            •control any variables: enzyme concentration, pH, volume of solution

        •measure how fast a substrate disappears

                  •the enzyme amylase catalyses the breakdown of starch to maltose

                  •use iodine solution to detect the starch

                  •time how long for the starch to disappear by regularly sampling the starch solution, and use the times to compare rates between different tests

                  •by adjusting the water bath temperature, you can see how the temperature affects the activity of amylase: you need to control all the variables